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KMID : 1161420130160110984
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Journal of Medicinal Food
2013 Volume.16 No. 11 p.984 ~ p.991
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In Vitro Study on the Antioxidant Activity of a Polyphenol-Rich Extract from Pinus brutia Bark and Its Fractions
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Cretu Elena
Karonen Maarit
Salminen Juha-Pekka
Mircea Cornelia
Trifan Adriana
Charalambous Christiana
Constantinou Andreas I.
Miron Anca
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Abstract
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A crude hydromethanolic extract from Pinus brutia bark and its fractions (diethyl ether, ethyl acetate, n-butanol, and aqueous fractions) were studied with regard to their phenolic content and antioxidant activities. The total phenolics and proanthocyanidins in each extract were quantified by spectrophotometric methods; the polyphenolic profile was analyzed by RP-HPLC-DAD-ESI-MS. All extracts were tested with regard to their ability to scavenge free radicals (ABTS radical cation, superoxide and hydroxyl radicals), reduce ferric ions, and inhibit 15-lipoxygenase. P. brutia bark extracts had high phenolic contents (303.79±7.34?448.90±1.39?mg/g). Except diethyl ether extract, all other extracts contained proanthocyanidins ranging from 225.79±3.94 to 250.40±1.44?mg/g. Several polyphenols were identified by RP-HPLC-DAD-ESI-MS: taxifolin in diethyl ether extract, a taxifolin-O-hexoside, catechin, procyanidin dimers, and trimers in ethyl acetate extract. Except diethyl ether extract, all other extracts were effective scavengers of superoxide and hydroxyl radicals (EC50=33.5±1.1?54.93±2.85 μg/mL and 0.47±0.06?0.6±0.0?mg/mL, respectively). All extracts had noticeable 15-lipoxygenase inhibitory effects (EC50=22.47±0.75?34.43±2.25 μg/mL). We conclude that P. brutia bark is very promising for the dietary supplements industry due to its high free radical scavenging and 15-lipoxygenase inhibitory effects.
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KEYWORD
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Calabrian pine, hydroxyl radical, 15-lipoxygenase, proanthocyanidins, RP-HPLC-DAD-ESI-MS, superoxide anion radical, taxifolin
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